IL12 Protein (AA 23-219) (Fc-His Tag)

Catalog No. ABIN7491453

Product Details

Target: IL12 (Interleukin 12 (IL12))

Protein Type: Recombinant

Protein Characteristics: AA 23-219

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL12 protein is labelled with Fc-His Tag.

Purpose: Recombinant Human IL12A Protein with C-terminal human Fc tag and Human IL12B Protein with C-terminal 6xHis tag

Specificity: IL12A (Arg23-Ser219) hFc (Glu99-Ala330)and IL12B (Ile23-Ser328) 6xHis tag

Characteristics: Extracellular Domain Protein

Purification: Purified from cell culture supernatant by affinity chromatography

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL12A &IL12B (IL12 Products)

Background: Interleukin 12 (IL12) is also known as p70, and is an interleukin that is naturally produced by dendritic cells, macrophages and human B-lymphoblastoid cells (NC-37) in response to antigenic stimulation.IL12 is a heterodimeric cytokine, containing IL-12A (p35) and IL-12B (p40). IL-12 is involved in the differentiation of naive T cells into Th1 cells. It is known as a T cell-stimulating factor, which can stimulate the growth and function of T cells. It stimulates the production of IFN-γ and TNF-a from T cells and NK cells, and reduces IL-4 mediated suppression of IFN-γ. IL-12 plays an important role in the activities of natural killer cells and T lymphocytes. IL-12 also has anti-angiogenic activity, which means it can block the formation of new blood vessels.

Molecular Weight: predicted molecular mass of 48.7 and 35.5 kDa after removal of the signal peptide.

UniProt: P29459, P29460

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome