PVRIG Protein (DYKDDDDK Tag)
Quick Overview for PVRIG Protein (DYKDDDDK Tag) (ABIN7491723)
Target
See all PVRIG ProteinsProtein Type
Origin
Source
Application
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Purification tag / Conjugate
- This PVRIG protein is labelled with DYKDDDDK Tag.
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Purpose
- Human PVRIG full length protein-synthetic nanodisc
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Characteristics
- Unlike other membrane scaffold protein (MSP) Nanodisc on the market, our synthetic Nanodisc can be prepared directly from the cells. The polymers used during this process have a dual function. It dissolves the cell membranes, like the detergent, and uses cellular phospholipids to form Nanodisc around the membrane proteins. The target protein embedded Nanodiscs can then be purified.
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Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 41-171) (Active) protein (hFc Tag)
PVRIG Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 41-171) (Active) protein (His tag)PVRIG Origin: Cynomolgus Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC Active
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 1-326) protein (His tag)custom-made PVRIG Origin: Human Host: HEK-293 Cells Recombinant > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (Active) protein (mFc Tag)PVRIG Origin: Human Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE. Active
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 35-165) (Active) protein (hFc-Avi Tag)PVRIG Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 35-165) (Active) protein (hFc-Avi Tag,Biotin)PVRIG Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by Bis-Tris PAGE Active
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 41-171) (Active) protein (His tag)PVRIG Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) protein (His tag)PVRIG Origin: Cynomolgus Host: Human Cells Recombinant >95 % as determined by reducing SDS-PAGE.
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 99-330) protein (Fc Tag)PVRIG Origin: Human Host: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining. AM, ADA, IA, ScA
Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG) (AA 38-326) protein (GST tag)PVRIG Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Comment
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Advantages:
- Highly purified membrane proteins
- High solubility in aqueous solutions
- High stability
- Proteins are in a native membrane environment and remain biologically active
- No detergent and can be used for cell-based assays
- No MSP backbone proteins
- Intolerant to acids and high concentrations of divalent metal ions
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Please see Certificate of Analysis for specific instructions of reconstitution
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Buffer
- Lyophilized from nanodisc solubilization buffer (20 mM Tris-HCl, 150 mM NaCl, pH 8.0). Normally 5 % - 8 % trehalose is added as protectants before lyophilization.
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Storage
- -20 °C,-80 °C
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Storage Comment
- Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.
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Expiry Date
- 12 months
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- PVRIG (Poliovirus Receptor-Related Immunoglobulin Domain Containing (PVRIG))
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Alternative Name
- PVRIG
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Background
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C7orf15, CD112R
Cell surface receptor for NECTIN2. May act as a coinhibitory receptor that suppresses T-cell receptor-mediated signals. Following interaction with NECTIN2, inhibits T-cell proliferation. Competes with CD226 for NECTIN2-binding. -
Molecular Weight
- The human full length PVRIG protein has a MW of 34.3 kDa
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UniProt
- Q6DKI7
Target
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