Pro BDNF Protein (Biotin)

Catalog No. ABIN7491800

This Recombinant Pro BDNF protein is expressed in Escherichia coli (E. coli).

Quick Overview for Pro BDNF Protein (Biotin) (ABIN7491800)

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), Live Cell Imaging (LCI), Cell Culture (CC)

Product Details

Purification tag / Conjugate: This Pro BDNF protein is labelled with Biotin.

Purpose: human proBDNF-Biotin

Sequence: MAPMKEANIR GQGGLAYPGV RTHGTLESVN GPKAGSRGLT SLADTFEHVI EELLDEDQKV RPNEENNKDA DLYTSRVMLS SQVPLEPPLL FLLEEYKNYL DAANMSMRVAGHSDPARRGEL SVCDSISEWV TAADKKTAVD MSGGTVTVLE KVPVSKGQLK QYFYETKCNP MGYTKEGCRG IDKRHWNSQC RTTQSYVRAL TMDSKKRIGW RFIRIDTSCV CTLTIKRGR (the prodomain is shown in bold, underlined are the double point mutations preventing cleavage)

Specificity: Toxin target: p75 neurotrophin receptor (p75NTR), Sortilin receptors

Characteristics: LC-Biotin. The extent of labeling is 1-3 Molecules of biotin per one molecule of human proBDNF, linked via primary amines.

Biological Activity Comment: proBDNF has been shown to be a pro-apoptotic ligand for sympathetic neurons expressing both p75 and sortilin.

Application Details

Application Notes: Antigen Preadsorption Control: 0.1 - 10 nM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handling Advice: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Storage: -20 °C

Storage Comment: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Target Details

Target: Pro BDNF (proBDNF) (Pro-Brain-Derived Neurotrophic Factor (proBDNF))

Alternative Name: proBDNF

Background: A biotinylated human proBDNF for sensitive detection of p75NTR, sortilin receptors, and the study of neuronal apoptosis and long-term depression (LTD)

Molecular Weight: ~52 kDa (dimer)