Cardiotrophin 1 Protein (CTF1)

Catalog No. ABIN7482110

This Recombinant Cardiotrophin 1 protein is produced in Escherichia coli (E. coli).

Quick Overview for Cardiotrophin 1 Protein (CTF1) (ABIN7482110)

Target: Cardiotrophin 1 (CTF1)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), Cell Culture (CC)

Purity: ≥98 % (HPLC)

Product Details

Purpose: Recombinant human Cardiotrophin-1 protein

Sequence: SRREGSLEDP QTDSSVSLLP HLEAKIRQTH SLAHLLTKYA EQLLQEYVQL QGDPFGLPSF SPPRLPVAGL SAPAPSHAGL PVHERLRLDA AALAALPPLL DAVCRRQAEL NPRAPRLLRR LEDAARQARA LGAAVEALLA ALGAANRGPR AEPPAATASA ASATGVFPAK VLGLRVCGLY REWLSRTEGD LGQLLPGGSA

Specificity: Toxin target: Leukemia Inhibitory Factor (LIF) receptor, ILST/gp130 receptors

Endotoxin Level: <0.1 EU per 1 μg of the protein by the LAL method.

Biological Activity Comment: Cardiotrophin-1 is a member of the neuropoietic cytokine family which enhances the survival of ciliary and dopaminergic neurons through the interaction with the gp130 receptor. Cardiotrophin-1 induces cardiac myocyte hypertrophy and has been shown to promote the survival of both embryonic and neonatal rat ventricular muscle cells1-3.

Application Details

Application Notes: Antigen Preadsorption Control: 10 - 100 nM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. For storage of dilute working solutions, we recommend adding a carrier protein (e.g., 0.1 % BSA) to your buffer to prevent material loss due to protein adsorption. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from sterile double distilled water (ddH2O).

Handling Advice: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Storage: -20 °C

Storage Comment: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Target Details

Target: Cardiotrophin 1 (CTF1)

Alternative Name: Cardiotrophin-1

Background: A recombinant human cytokine that promotes cardiac myocyte hypertrophy and survival, and acts as a neurotrophic factor via the gp130/LIFR receptor complex

Molecular Weight: 21.1 kDa

UniProt: Q16619

Pathways: JAK-STAT Signaling