CNTF Protein

Catalog No. ABIN7491826

Recombinant CNTF protein expressed in Escherichia coli (E. coli).

Quick Overview for CNTF Protein (ABIN7491826)

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), Cell Culture (CC)

Purity: ≥98 % (HPLC)

Product Details

Purpose: Recombinant rat CNTF protein

Sequence: AFAEQTPLTL HRRDLCSRSI WLARKIRSDL TALMESYVKH QGLNKNINLD SVDGVPVAST DRWSEMTEAE RLQENLQAYR TFQGMLTKLL EDQRVHFTPT EGDFHQAIHT LMLQVSAFAY QLEELMVLLE QKIPENEADG MPATVGDGGL FEKKLWGLKV LQELSQWTVR SIHDLRVISS HQMGISALES HYGAKDKQM

Specificity: Toxin target: Leukemia Inhibitory Factor (LIF) receptor, gp130, CNTFR

Endotoxin Level: <0.1 EU per 1 μg of the protein by the LAL method.

Biological Activity Comment: CNTF is a potent neurotrophic factor that was originally characterized as a survival factor for chick ciliary neurons. CNTF supports growth and survival of DRG, motor and sympathetic neurons1-4.

Application Details

Application Notes: Antigen Preadsorption Control: EC50 = ~2.2 pM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. For storage of dilute working solutions, we recommend adding a carrier protein (e.g., 0.1 % BSA) to your buffer to prevent material loss due to protein adsorption. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handling Advice: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Storage: -20 °C

Storage Comment: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Target Details

Target: CNTF (Ciliary Neurotrophic Factor (CNTF))

Alternative Name: CNTF

Background: A potent survival factor for neurons and oligodendrocytes

Molecular Weight: 22.7 kDa

UniProt: P20294

Pathways: JAK-STAT Signaling