NGF 2.5S (Active) Protein

Catalog No. ABIN7887294

Native NGF 2.5S protein expressed in Mouse.

Quick Overview for NGF 2.5S (Active) Protein (ABIN7887294)

Target: NGF 2.5S

Protein Type: Native

Biological Activity: Active

Origin: Mouse

Source: Mouse

Application: Cell Culture (CC), Cytotoxicity Test (CyTox), ELISA

Purity: >95 % (HPLC)

Product Details

Purpose: Native mouse NGF 2.5S protein (>95%)

Specificity: Toxin target: p75 neurotrophin receptor (p75NTR), TrkA receptors

Biological Activity Comment: NGF is purified in three forms: the 7S, 2.5S and β. The 2.5S form is 9 amino acids shorter than the β form because of proteolysis that occurs during the purification process1. NGF has been shown to regulate neuronal survival, development, function and plasticity2. The biological effects of NGF are mediated by two receptors: TrkA, which is specific for NGF, and p75NTR, which binds all the neurotrophins3.

Application Details

Application Notes: Antigen Preadsorption Control: EC50 = 0.7 nM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. Reconstitute in sterile, high-purity water at a concentration of at least 0.1 mg/mL. For long-term storage, aliquot the solution into small, single-use vials and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual acetate.

Handling Advice: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Storage: -20 °C

Storage Comment: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Target Details

Target: NGF 2.5S

Background: A native mouse neurotrophin purified from submaxillary glands that promotes neuronal survival and neurite outgrowth via TrkA and p75NTR receptors

Molecular Weight: 26 kDa

CAS-No: 93928-24-6