IL-21 Protein (His tag)

Catalog No. ABIN7482578

This Recombinant IL-21 protein is produced in HEK-293 Cells.

Quick Overview for IL-21 Protein (His tag) (ABIN7482578)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: 90 %

Product Details

Purification tag / Conjugate: This IL-21 protein is labelled with His tag.

Purpose: Mouse IL-21 Protein, His Tag

Sequence: Pro 25 - Ser 146

Characteristics: Mouse IL-21 Protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Pro 25 - Ser 146 (Accession # Q9ES17).

Endotoxin Level: 1.0 EU per μg

Application Details

Comment:

This protein carries a polyhistidine tag at the C-terminus. (10xHis) The protein has a calculated MW of 16.1 kDa. The protein migrates as 18 kDa and 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: PBS, pH 7.4

Storage: -20 °C

Storage Comment: -20°C

Target Details

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL-21

Background: Interleukin-21 (IL-21) is a secreted protein which belongs to the IL-15 / IL-21 family. Interleukin-21 / IL-21 belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL21R) and the common cytokine receptor gamma chain (gamma(C)). Interleukin-21 / IL-21 impacts a number of cell types, including CD8+ memory T cells, NK cells and subsets of CD4 memory T cells. The IL-21R is widely distributed on lympho-haematopoietic cells. IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.

Molecular Weight: 16.1 kDa

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response