BCMA Protein (AA 1-53) (His tag)

Catalog No. ABIN7504239

This Recombinant BCMA protein is produced in HEK-293 Cells.

Quick Overview for BCMA Protein (AA 1-53) (His tag) (ABIN7504239)

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Protein Type: Recombinant

Biological Activity: Active

Origin: Cynomolgus, Rhesus Monkey

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 1-53

Purification tag / Conjugate: This BCMA protein is labelled with His tag.

Purpose: Cynomolgus/Rhesus macaque BCMA/TNFRSF17 Protein

Sequence: Met1-Ala53

Characteristics: Recombinant Cynomolgus/Rhesus macaque BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag at the C-terminus.It contains Met1-Ala53.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Cynomolgus BAFF (Trimer), His Tag captured on CM5 Chip via streptavidin can bind Cynomolgus BCMA, His Tag with an affinity constant of 1.40 μM as determined in SPR assay (Biacore T200).,Immobilized Cynomolgus BCMA, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Biotinylated Human APRIL (Trimer), His Tag with the EC50 of 15.8 ng/ml determined by ELISA (QC Test).,Immobilized Cynomolgus BCMA, His Tag at 0.5 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-BCMA Antibody, hFc Tag with the EC50 of 3.2 ng/ml determined by ELISA.,Cynomolgus/Rhesus macaque BCMA on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,Loaded Biotinylated Human APRIL (Trimer), His-Avi Tag on Streptavidin-Biosensor can bind Cynomolgus BCMA, His Tag with an affinity constant of 5.75 nM as determined in BLI assay (Gator® Prime).,Loaded Anti-BCMA antibody on ProA-Biosensor can bind Cynomolgus BCMA, His Tag with an affinity constant of 8.99 nM as determined in BLI assay (Gator® Prime).,Loaded Biotinylated Cynomolgus BAFF (Trimer), His-Avi-Flag Tag on Streptavidin-Biosensor, can bind Cynomolgus BCMA, His Tag with an affinity constant of 1.69 μM as determined in BLI assay (Gator).,The purity of Cynomolgus/Rhesus macaque BCMA is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3 months after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: BCMA (TNFRSF17) (Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))

Alternative Name: BCMA

Background: B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene. TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).

Molecular Weight: 7.10 kDa. Due to glycosylation, the protein migrates to 13-23 kDa based on Bis-Tris PAGE result.

UniProt: A0A2K5UD97