BDNF Protein (AA 129-247)

Catalog No. ABIN7504243

This Recombinant BDNF protein is expressed in Escherichia coli (E. coli).

Quick Overview for BDNF Protein (AA 129-247) (ABIN7504243)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human, Rat, Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE

Product Details

Protein Characteristics: AA 129-247

Purpose: Human/Mouse/Rat BDNF Protein

Sequence: His129-Arg247(Human) / His131-Arg249(Mouse/Rat)

Characteristics: Recombinant Human/Mouse/Rat BDNF Protein is expressed from E.coli without tag. It contains His129-Arg247(Human) / His131-Arg249(Mouse/Rat).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 0.01 EU per μg by the LAL method.

Biological Activity Comment: Immobilized Human/Mouse/Rat BDNF, No Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human TrkB, His Tag with the EC50 of 0.39 ug/ml determined by ELISA (QC Test).,Human/Mouse/Rat BDNF on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background: The brain-derived neurotrophic factor (BDNF) is a secretory growth factor that promotes neuronal proliferation and survival, synaptic plasticity and long-term potentiation in the central nervous system. Brain-derived neurotrophic factor biosynthesis and secretion are chrono-topically regulated processes at the cellular level, accounting for specific localizations and functions.

Molecular Weight: 13.51 kDa. The protein migrates to 15-19 kDa based on Bis-Tris PAGE result.

UniProt: P23560

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity