CEACAM8 Protein (AA 35-319) (Biotin,His-Avi Tag)

Catalog No. ABIN7504299

This Recombinant CEACAM8 protein is produced in HEK-293 Cells.

Quick Overview for CEACAM8 Protein (AA 35-319) (Biotin,His-Avi Tag) (ABIN7504299)

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: AA 35-319

Purification tag / Conjugate: This CEACAM8 protein is labelled with Biotin,His-Avi Tag.

Purpose: Biotinylated Human CEACAM-8/CD66b Protein

Sequence: Gln35-Ser319

Characteristics: Recombinant Biotinylated Human CEACAM-8/CD66b Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln35-Ser319.

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Biotinylated Human CEACAM-8, His Tag immobilized on CM5 Chip can bind Human CEACAM-6, His Tag with an affinity constant of 6.13 uM as determined in SPR assay (Biacore T200).,Biotinylated Human CEACAM-8 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Biotinylated Human CEACAM-8 is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: CEACAM8 (Carcinoembryonic Antigen-Related Cell Adhesion Molecule 8 (CEACAM8))

Alternative Name: CEACAM-8

Background: Eosinophils and their products are likely important in the pathophysiology of allergic diseases, such as bronchial asthma, and in host immunity to parasitic organisms. CD66b (CEACAM8, CGM6, NCA-95) is a single chain, GPI-anchored, highly glycosylated protein belonging to the carcinoembryonic Ag supergene family. CD66b is an activation marker for human granulocytes.

Molecular Weight: 34.3 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

NCBI Accession: NP_001807