FYN Protein (AA 82-246) (His tag)

Catalog No. ABIN7505014

Product Details

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Protein Type: Recombinant

Protein Characteristics: AA 82-246

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This FYN protein is labelled with His tag.

Sequence: Thr82-Cys246

Characteristics: A DNA sequence encoding theHuman Fyn protein (P06241) (Thr82-Cys246) was expressed with a N-His.

Purity: >85 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: FYN (FYN Oncogene Related To SRC, FGR, YES (FYN))

Alternative Name: Fyn (FYN Products)

Synonyms: im:6908851 Protein, si:dkey-119m7.5 Protein, zgc:109996 Protein, c-fyn Protein, cfyn-a Protein, SLK Protein, SYN Protein, p59-FYN Protein, PKC Protein, fyn Protein, zgc:86720 Protein, AI448320 Protein, AW552119 Protein, FYN proto-oncogene, Src family tyrosine kinase Protein, FYN proto-oncogene, Src family tyrosine kinase S homeolog Protein, FYN proto-oncogene, Src family tyrosine kinase b Protein, FYN proto-oncogene, Src family tyrosine kinase L homeolog Protein, FYN proto-oncogene, Src family tyrosine kinase a Protein, Fyn proto-oncogene Protein, FYN Protein, fyn.S Protein, fynb Protein, fyn.L Protein, Fyn Protein, fyna Protein

Background:

Background: Non-receptor tyrosine-protein kinase that plays a role in many biological processes including regulation of cell growth and survival, cell adhesion, integrin-mediated signaling, cytoskeletal remodeling, cell motility, immune response and axon guidance. Inactive FYN is phosphorylated on its C-terminal tail within the catalytic domain. Following activation by PKA, the protein subsequently associates with PTK2/FAK1, allowing PTK2/FAK1 phosphorylation, activation and targeting to focal adhesions. Involved in the regulation of cell adhesion and motility through phosphorylation of CTNNB1 (beta-catenin) and CTNND1 (delta-catenin). Regulates cytoskeletal remodeling by phosphorylating several proteins including the actin regulator WAS and the microtubule-associated proteins MAP2 and MAPT. Promotes cell survival by phosphorylating AGAP2/PIKE-A and preventing its apoptotic cleavage. Participates in signal transduction pathways that regulate the integrity of the glomerular slit diaphragm (an essential part of the glomerular filter of the kidney) by phosphorylating several slit diaphragm components including NPHS1, KIRREL1 and TRPC6. Plays a role in neural processes by phosphorylating DPYSL2, a multifunctional adapter protein within the central nervous system, ARHGAP32, a regulator for Rho family GTPases implicated in various neural functions, and SNCA, a small pre-synaptic protein. Participates in the downstream signaling pathways that lead to T-cell differentiation and proliferation following T-cell receptor (TCR) stimulation. Phosphorylates PTK2B/PYK2 in response to T-cell receptor activation. Also participates in negative feedback regulation of TCR signaling through phosphorylation of PAG1, thereby promoting interaction between PAG1 and CSK and recruitment of CSK to lipid rafts. CSK maintains LCK and FYN in an inactive form. Promotes CD28-induced phosphorylation of VAV1. In mast cells, phosphorylates CLNK after activation of immunoglobulin epsilon receptor signaling.

Synonym: Proto-oncogene Syn,Proto-oncogene c-Fyn,Src-like kinase,SLK,p59-Fyn

Molecular Weight: 19 kDa

UniProt: P06241

Pathways: JAK-STAT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Feeding Behaviour, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Activated T Cell Proliferation, Thromboxane A2 Receptor Signaling