MCM3 Protein (AA 41-300) (His tag)

Catalog No. ABIN7505054

This Recombinant MCM3 protein is expressed in Escherichia coli (E. coli).

Quick Overview for MCM3 Protein (AA 41-300) (His tag) (ABIN7505054)

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 41-300

Purification tag / Conjugate: This MCM3 protein is labelled with His tag.

Sequence: Ser 41-Ala 300

Characteristics: A DNA sequence encoding the Human MCM3 protein (P25205) (Ser 41-Ala 300) was expressed with N-His&C-His tag.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Alternative Name: MCM3

Background:

Abbreviation: MCM3

Target Synonym: MCM3,P1 Protein,P1-MCM3,P1.h,p102,P102 protein,MCM 3,mcm3

Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with mCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight:

Calculated MW: 28.49 kDa

Observed MW: 35 kDa

UniProt: P25205

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA