ALPL Protein (AA 1-502) (His tag)

Catalog No. ABIN7505728

This Recombinant ALPL protein is produced in HEK-293 Cells.

Quick Overview for ALPL Protein (AA 1-502) (His tag) (ABIN7505728)

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 80 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-502

Purification tag / Conjugate: This ALPL protein is labelled with His tag.

Sequence: Met1-Ser502

Characteristics: A DNA sequence encoding theMouse ALPL protein (P09242) (Met1-Ser502) was expressed with a C-His.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: ALPL (Alkaline Phosphatase, Liver/bone/kidney (ALPL))

Alternative Name: ALPL

Background:

Abbreviation: ALPL

Target Synonym: AP-TNAP,TNAP,TNSALP,Alkaline phosphatase 2,Alkaline phosphatase liver/bone/kidney isozyme,Phosphoamidase,Phosphocreatine phosphatase

Background: Alkaline phosphatase (ALPL) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. As the name suggests, alkaline phosphatases are most effective in an alkaline environment. It is sometimes used synonymously as basic phosphatase. Alkaline phosphatases (APs) are ubiquitous in many species, from bacteria to human. Four genes encode AP isoenzymes in humans and rodents. Three AP genes are expressed in a tissue-specific manner (i.e., placental, embryonic, and intestinal AP isoenzymes). Expression of the fourth AP gene is nonspecific to a single tissue and is especially abundant in bone, liver, and kidney. This isoenzyme is also called tissue-nonspecific alkaline phosphatase (TNAP). The enzyme tissue non-specific alkaline phosphatase (TNAP) belongs to the ectophosphatase family. TNAP is present in large amounts in bone in which it plays a role in mineralization.

Molecular Weight:

Calculated MW: 52 kDa

Observed MW: 70 kDa

UniProt: P09242