CD276 Protein (CD276) (His tag)

Catalog No. ABIN7536181

This Recombinant CD276 protein is expressed in HEK-293 Cells.

Quick Overview for CD276 Protein (CD276) (His tag) (ABIN7536181)

Target: CD276

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 97 % by SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD276 protein is labelled with His tag.

Purpose: Active Recombinant Mouse CD276 Protein

Sequence: VEVQVSEDPV VALVDTDATL RCSFSPEPGF SLAQLNLIWQ LTDTKQLVHS FTEGRDQGSA YSNRTALFPD LLVQGNASLR LQRVRVTDEG SYTCFVSIQD FDSAAVSLQV AAPYSKPSMT LEPNKDLRPG NMVTITCSSY QGYPEAEVFW KDGQGVPLTG NVTTSQMANE RGLFDVHSVL RVVLGANGTY SCLVRNPVLQ QDAHGSVTIT GQPLTF

Specificity: Val 29 - Phe 244

Sterility: 0.22 μm filtered

Endotoxin Level: <0.1EU/μg

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized Mouse CD276 at 2 μg/mL (100 μL/well) can bind CD276 Rabbit pAb with a linear range of 1-274 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: CD276

Alternative Name: CD276

Background:

Description: B7-H3 is a member of the B7 family of immune regulatory ligands that is thought to attenuate peripheral immune responses through co-inhibition. It plays an important role in adaptive immune responses, and was shown to either promote or inhibit T-cell responses in various experimental systems. B7-H3 may play an important role in muscle-immune interactions, providing further evidence of the active role of muscle cells in local immunoregulatory processes. B7-H3 is a novel protein structurally related to the B7 family of ligands by the presence of a single set of immunoglobulin-V-like and immunoglobulin-C-like (VC) domains. Previous studies have correlated its overexpression with poor prognosis and decreased tumor-infiltrating lymphocytes in various carcinomas including uterine endometrioid carcinomas, and mounting evidence supports an immuno-inhibitory role in ovarian cancer prognosis. Recently, B7-H3 expression has been reported in several human cancers indicating an additional function of B7-H3 as a regulator of antitumor immunity.

Name: B7h3, B7RP-2, 6030411F23Rik,CD276

Gene ID: 102657

UniProt: Q8VE98

Pathways: Cancer Immune Checkpoints