CD47 Protein (CD47) (Fc Tag)

Catalog No. ABIN7536134

This Recombinant CD47 protein is produced in HEK-293 Cells.

Quick Overview for CD47 Protein (CD47) (Fc Tag) (ABIN7536134)

Target: CD47

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purity: > 97 % by SDS-PAGE.

Product Details

Purification tag / Conjugate: This CD47 protein is labelled with Fc Tag.

Purpose: Active Recombinant Rat CD47 Protein

Sequence: QLLLSKVKSV EFTSCNDTVV IPCKVLNVEA QSTDEMFVKW KLNKSYIFIY DGNKNSTTRE QNFTSAKISV SDLLKGIASL TMDTHEAVVG NYTCEVTELS REGKTVIELK NRPVSWFSTN EK

Specificity: Gln 19 - Lys 140

Sterility: 0.22 μm filtered

Endotoxin Level: <0.1EU/μg

Biological Activity Comment: Measured by its binding ability in a functional ELISA.Immobilized Human CD172a at 2 μg/mL (100 μL/well) can bind Human CD47 with a linear range of 19.5-3491.5ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: CD47

Alternative Name: CD47

Background:

Description: CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.

Name: Itgp,CD47

Gene ID: 29364

UniProt: P97829