HGF Protein (His tag)

Catalog No. ABIN7520064

Recombinant HGF protein expressed in HEK-293 Cells.

Quick Overview for HGF Protein (His tag) (ABIN7520064)

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: HEK-293 Cells

Purity: > 92 % by SDS-PAGE.

Product Details

Purification tag / Conjugate: This HGF protein is labelled with His tag.

Purpose: Recombinant Rat Hepatocyte growth factor/HGF Protein

Sequence: QKKRRNTLHE FKKSAKTTLT KEDPLVKIKT KKVNSADECA NRCIRNKGFP FTCKAFVFDK SRKRCYWYPF NSMSSGVKKG FGHEFDLYEN KDYIRNCIIG KGGSYKGTVS ITKSGIKCQP WNSMIPHEHS FLPSSYRGKD LQENYCRNPR GEEGGPWCFT SNPEVRYEVC DIPQCSEVEC MTCNGESYRG PMDHTESGKT CQRWDQQTPH RHKFLPERYP DKGFDDNYCR NPDGKPRPWC YTLDPDTPWE YCAIKMCAHS AVNETDVPME TTECIKGQGE GYRGTTNTIW NGIPCQRWDS QYPHKHDITP ENFKCKDLRE NYCRNPDGAE SPWCFTTDPN IRVGYCSQIP KCDVSSGQDC YRGNGKNYMG NLSKTRSGLT CSMWDKNMED LHRHIFWEPD ASKLTKNYCR NPDDDAHGPW CYTGNPLVPW DYCPISRCEG DTTPTIVNLD HPVISCAKTK QLRVVNGIPT QTTVGWMVSL KYRNKHICGG SLIKESWVLT ARQCFPARNK DLKDYEAWLG IHDVHERGEE KRKQILNISQ LVYGPEGSDL VLLKLARPAI LDNFVSTIDL PSYGCTIPEK TTCSIYGWGY TGLINADGLL RVAHLYIMGN EKCSQHHQGK VTLNESELCA GAEKIGSGPC EGDYGGPLIC EQHKMRMVLG VIVPGRGCAI PNRPGIFVRV AYYAKWIHKV ILTYKL

Specificity: Gln 33-Leu728

Sterility: 0.22 μm filtered

Endotoxin Level: <0.001EU/μg

Biological Activity Comment: Measured in a cell proliferation assay using Mv.1.lu cells. The ED₅₀ for this effect is 1.6-6.2 ng/mL, corresponding to a specific activity of 1.6x10⁵~6.3x10⁵ units/mg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Concentration: 0.67 mg/mL

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: HGF (Hepatocyte Growth Factor (Hepapoietin A, Scatter Factor) (HGF))

Alternative Name: Hepatocyte growth factor/HGF

Background:

Description: Hepatocyte growth factor/scatter factor (HGF/SF) is a paracrine cellular growth, motility and morphogenicfactor. It belongs to the peptidase S1 family and Plasminogen subfamily, contains 4 kringle domains, 1 PANdomain and 1 peptidase S1 domain. HGF regulates cell growth, cell motility, and morphogenesis by activatinga tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. HGF is secreted bymesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability tostimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis,and tissue regeneration.

Name: HPTA,Hepatocyte growth factor,HGF

Gene ID: 24446

UniProt: P17945

Pathways: RTK Signaling, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor