Oncostatin M Protein (OSM)

Catalog No. ABIN7520374

This Recombinant Oncostatin M protein is produced in Escherichia coli (E. coli).

Quick Overview for Oncostatin M Protein (OSM) (ABIN7520374)

Target: Oncostatin M (OSM)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % by SDS-PAGE.

Product Details

Purpose: Active Recombinant Human Oncostatin-M/OSM Protein

Sequence: AAIGSCSKEY RVLLGQLQKQ TDLMQDTSRL LDPYIRIQGL DVPKLREHCR ERPGAFPSEE TLRGLGRRGF LQTLNATLGC VLHRLADLEQ RLPKAQDLER SGLNIEDLEK LQMARPNILG LRNNIYCMAQ LLDNSDTAEP TKAGRGASQP PTPTPASDAF QRKLEGCRFL HGYHRFMHSV GRVFSKWGES PNRSRR

Specificity: Ala26-Arg221

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.01 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is typically 0.8-3.2 ng/mL, corresponding to a specific activity of 3.12x10⁵-1.25x10⁶units/mg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 150 mM NaCl, 5 % glycerol, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: Oncostatin M (OSM)

Alternative Name: Oncostatin-M/OSM

Background:

Description: This protein is a member of the leukemia inhibitory factor/oncostatin-M (LIF/OSM) family. The preproprotein is proteolytically processed to generate the mature protein. This protein is a secreted cytokine and growth regulator that inhibits the proliferation of a number of tumor cell lines. This protein also regulates the production of other cytokines, including interleukin 6, granulocyte-colony stimulating factor and granulocyte-macrophage colony stimulating factor in endothelial cells.

Name: OSM, oncostatin-M

Gene ID: 5008

UniProt: P13725

Pathways: JAK-STAT Signaling, Negative Regulation of Hormone Secretion