SARS-CoV Spike Protein (RBD) (His tag)

Catalog No. ABIN7520456

This Recombinant SARS-CoV Spike protein is expressed in HEK-293 Cells.

Quick Overview for SARS-CoV Spike Protein (RBD) (His tag) (ABIN7520456)

Target: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Protein Type: Recombinant

Biological Activity: Active

Origin: SARS Coronavirus (SARS-CoV)

Source: HEK-293 Cells

Purity: > 95 % by SDS-PAGE.

Product Details

Protein Characteristics: RBD

Purification tag / Conjugate: This SARS-CoV Spike protein is labelled with His tag.

Purpose: Active Recombinant SARS-CoV Spike RBD Protein

Sequence: RVVPSGDVVR FPNITNLCPF GEVFNATKFP SVYAWERKKI SNCVADYSVL YNSTFFSTFK CYGVSATKLN DLCFSNVYAD SFVVKGDDVR QIAPGQTGVI ADYNYKLPDD FMGCVLAWNT RNIDATSTGN YNYKYRYLRH GKLRPFERDI SNVPFSPDGK PCTPPALNCY WPLNDYGFYT TTGIGYQPYR VVVLSFELLN APATVCGPKL STDLIKNQCV NF

Specificity: Arg306-Phe527

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized SARS-CoV Spike RBD at 2μg/mL (100μL/well) can bind Human ACE2 with a linear range of 0.1-5.18 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS,300 mM NaCl, pH 7.4.or Supplied as a 0.22 μm filtered solution in PBS,300 mM NaCl, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.|After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.|or This product is stable at ≤ -70°C for up to 6 months from the date of receipt. |For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Target Details

Target: SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))

Alternative Name: SARS-CoV Spike

Target Type: Viral Protein

Background: Spike,Spike RBD,Spike S1

Gene ID: 1489668

UniProt: P59594