SARS-CoV Spike Protein (RBD) (His tag)
> 95 % as determined by SEC-MALS. ELISA, SDS
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- Target See all SARS-CoV Spike (SARS-CoV S) Proteins
- SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))
- Protein Type
- Recombinant
- Protein Characteristics
- RBD
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Origin
- SARS Coronavirus (SARS-CoV)
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This SARS-CoV Spike protein is labelled with His tag.
- Application
- ELISA, SDS-PAGE (SDS)
- Purpose
- SARS S protein RBD, His Tag (MALS verified)
- Sequence
- AA 306-527
- Characteristics
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SARS S protein RBD, His Tag is expressed from human 293 cells (HEK293). It contains AA Arg 306 - Phe 527 (Accession # AAP13567.1). Predicted N-terminus: Arg 306
This protein carries a polyhistidine tag at the C-terminus. - Purity
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> 90 % as determined by SDS-PAGE.
> 95 % as determined by SEC-MALS. - Sterility
- 0.22 μm filtered
- Endotoxin Level
- Less than 1.0 EU per μg by the LAL method.
- Top Product
- Discover our top product SARS-CoV S Protein
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Buffer
- PBS, pH 7.4
- Handling Advice
- Please avoid repeated freeze-thaw cycles.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
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For long term storage, the product should be stored at lyophilized state at -20°C or lower.
This product is stable after storage at:
4-8°C for 12 months in lyophilized state,
-70°C for 3 years under sterile conditions after reconstitution.
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An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).
: "
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An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).
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- Target
- SARS-CoV Spike (SARS-CoV S) (SARS-Coronavirus Spike Protein (SARS-CoV S))
- Alternative Name
- SARS-Coronavirus Spike Protein (SARS-CoV S Products)
- Target Type
- Viral Protein
- Background
- It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- Molecular Weight
- 26.9 kDa
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