IRF9 Protein (AA 1-393) (His tag)

Catalog No. ABIN7548540

Recombinant IRF9 protein expressed in HEK-293 Cells.

Quick Overview for IRF9 Protein (AA 1-393) (His tag) (ABIN7548540)

Target: IRF9 (Interferon Regulatory Factor 9 (IRF9))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-393

Purification tag / Conjugate: This IRF9 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant IRF9 Protein expressed in mammalian cells.

Sequence: MASGRARCTR KLRNWVVEQV ESGQFPGVCW DDTAKTMFRI PWKHAGKQDF REDQDAAFFK AWAIFKGKYK EGDTGGPAVW KTRLRCALNK SSEFKEVPER GRMDVAEPYK VYQLLPPGIV SGQPGTQKVP SKRQHSSVSS ERKEEEDAMQ NCTLSPSVLQ DSLNNEEEGA SGGAVHSDIG SSSSSSSPEP QEVTDTTEAP FQGDQRSLEF LLPPEPDYSL LLTFIYNGRV VGEAQVQSLD CRLVAEPSGS ESSMEQVLFP KPGPLEPTQR LLSQLERGIL VASNPRGLFV QRLCPIPISW NAPQAPPGPG PHLLPSNECV ELFRTAYFCR DLVRYFQGLG PPPKFQVTLN FWEESHGSSH TPQNLITVKM EQAFARYLLE QTPEQQAAIL SLV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: IRF9 (Interferon Regulatory Factor 9 (IRF9))

Alternative Name: IRF9

Background: Interferon regulatory factor 9 (IRF-9) (IFN-alpha-responsive transcription factor subunit) (ISGF3 p48 subunit) (Interferon-stimulated gene factor 3 gamma) (ISGF-3 gamma) (Transcriptional regulator ISGF3 subunit gamma),FUNCTION: Transcription factor that plays an essential role in anti-viral immunity. It mediates signaling by type I IFNs (IFN-alpha and IFN-beta). Following type I IFN binding to cell surface receptors, Jak kinases (TYK2 and JAK1) are activated, leading to tyrosine phosphorylation of STAT1 and STAT2. IRF9/ISGF3G associates with the phosphorylated STAT1:STAT2 dimer to form a complex termed ISGF3 transcription factor, that enters the nucleus. ISGF3 binds to the IFN stimulated response element (ISRE) to activate the transcription of interferon stimulated genes, which drive the cell in an antiviral state. {ECO:0000269|PubMed:30143481}.

Molecular Weight: 43.7 kDa

UniProt: Q00978

AlphaFold: Q00978

Pathways: JAK-STAT Signaling, Interferon-gamma Pathway, Hepatitis C