PUMA Protein (AA 1-261) (His tag)

Catalog No. ABIN7552658

This Recombinant PUMA protein is expressed in HEK-293 Cells.

Quick Overview for PUMA Protein (AA 1-261) (His tag) (ABIN7552658)

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-261

Purification tag / Conjugate: This PUMA protein is labelled with His tag.

Purpose: Predefined custom protein recombinant BBC3 Protein expressed in mammalian cells.

Sequence: MKFGMGSAQA CPCQVPRAAS TTWVPCQICG PRERHGPRTP GGQLPGARRG PGPRRPAPLP ARPPGALGSV LRPLRARPGC RPRRPHPAAR CLPLRPHRPT RRHRRPGGFP LAWGSPQPAP RPAPGRSSAL ALAGGAAPGV ARAQRPGGSG GRSHPGGPGS PRGGGTVGPG DRGPAAADGG RPQRTVRAAE TRGAAAAPPL TLEGPVQSHH GTPALTQGPQ SPRDGAQLGA CTRPVDVRDS GGRPLPPPDT LASAGDFLCT M Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))

Alternative Name: BBC3

Background: Bcl-2-binding component 3, isoforms 3/4 (JFY-1) (p53 up-regulated modulator of apoptosis),FUNCTION: [Isoform 3]: Does not affect cell growth. {ECO:0000269|PubMed:11463392}.

Molecular Weight: 26.5 kDa

UniProt: Q96PG8

AlphaFold: Q96PG8

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity