Pejvakin Protein (AA 1-352) (His tag)

Catalog No. ABIN7556960

This Recombinant Pejvakin protein is expressed in HEK-293 Cells.

Quick Overview for Pejvakin Protein (AA 1-352) (His tag) (ABIN7556960)

Target: Pejvakin (DFNB59) (Deafness, Autosomal Recessive 59 (DFNB59))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-352

Purification tag / Conjugate: This Pejvakin protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Pjvk Protein expressed in mammalian cells.

Sequence: MFAAATKSFV KQVGDGGRLV PVPSLSEADK YQPLSLVVKK KRCFLFPRCK FTSTPFTLKD ILLGDREISA GISSYQLLNY EDESDVSLYG RRSNHIVNDV GINVTGSDSI AVKASFGVVT KHEVEVSTLL KEITARKINF DHSLIRQSRS SRKAVLCVVM ESIRTTRQCS LSVHAGIRGE AMRFHFMDEQ NPKGREKAIV FPAHTTIAFS VFELFIYLDG AFDICVTSVS KGGFEREETT TFAMFYRLRN ILFERNRRVM DAISRSQLYL DDLFSDFYDK PLSMTDISLK EGTHIRVNLL NHNIPKGPCI LCGMGNLKRE TVYGCFQCSV DGVKYVRLHA VPCFDIWHKR MK Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: Pejvakin (DFNB59) (Deafness, Autosomal Recessive 59 (DFNB59))

Alternative Name: Pjvk

Background: Pejvakin (Protein sirtaki),FUNCTION: Peroxisome-associated protein required to protect auditory hair cells against noise-induced damage (PubMed:26544938, PubMed:30936319). Acts by regulating noise-induced peroxisome proliferation in auditory hair cells and neurons, and promoting autophagic degradation of damaged peroxisomes (pexophagy) (PubMed:26544938, PubMed:30936319). Noise overexposure increases reactive oxygen species (ROS) levels, causing oxidative damage to auditory hair cells and resulting in hearing loss (PubMed:30936319). PJVK acts as a ROS sensor that recruits the autophagy machinery to trigger pexophagy of peroxisomes damaged by oxidative stress (PubMed:30936319). In addition to pexophagy, also required to promote peroxisome proliferation in response to sound overstimulation (PubMed:26544938, PubMed:30936319). {ECO:0000269|PubMed:26544938, ECO:0000269|PubMed:30936319}.

Molecular Weight: 39.9 kDa

UniProt: Q0ZLH2

AlphaFold: Q0ZLH2

Pathways: Sensory Perception of Sound