GFR Protein (AA 1-814) (His tag)

Catalog No. ABIN7559086

This Recombinant GFR protein is produced in HEK-293 Cells.

Quick Overview for GFR Protein (AA 1-814) (His tag) (ABIN7559086)

Target: GFR (RAPGEF5) (Rap Guanine Nucleotide Exchange Factor (GEF) 5 (RAPGEF5))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-814

Purification tag / Conjugate: This GFR protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Rapgef5 Protein expressed in mammalian cells.

Sequence: MTTGDCQTLS RRISNPYLEH SPSQIYGENS SCAGRALRNI IILQAADLVK DRVNLKGFYR RSCVGSELVD WLLEHCPFVQ CRSMAIGVWQ LLLDMGIMSS VDQHLYFQDN YVFYQFSSDE CSYLYCEFER EEEWQKGVKL LLELVHLIPA RAGICDLSHQ KTEDSEESSD EILARLTSAV QRELAAVIAL KARKSAIEQD DENADKHVTV TEANNGPDPQ AGVMCKLQER DDIGRIELVH KLARENCQFL QTEKKESEKL EQQDDEVTMV QVKEQGQSVL VLKKVASCGP APTSGSAEND ARYVVVSGTP EKILEHLLND LHLAEVQHKE TETLLDDFLL TYTVFMTTDD LCQALLRHYS AKKYQGEEEN SDVPCRKRKV LHLVSQWISL YKDWLHEDEH SKMFLKTIYR NVLDDVYEYP ILEKELKEFQ KILGVYRRHT VDEYSPQKKN KALFHQFSLK ENWLQHRGTV AETEEIFCHV YITEHSYISV KAKVSSTAQE ILKVVAEKLQ RAEEDLALVA ITFSGEKHEF QPNDLAISKS LEASGRIYVY RKDLADTLNP LAENEESQQR SMRILGMNTW DLALELMSFD WSLFNSIHEQ ELIYFTFSRQ GNGENTVNLS LLLQRCNEVQ LWVATEILLC SQLGKRVQLV KKFIKIAAHC KAQQNLNSFF AIVMGLNTAS VSRLSQTWEK IPGKFKKLFS ELESLTDPSL NHKAYRDAFK KMKPPKIPFM PLLLKDVTFI HEGNKTFLDN LVNFEKLHMI ADTVRTLRHC RTNQFGSDVS PKEQQELKSY VNHLYVIDSQ QALFELSHRL EPRA Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: GFR (RAPGEF5) (Rap Guanine Nucleotide Exchange Factor (GEF) 5 (RAPGEF5))

Alternative Name: Rapgef5

Background: Rap guanine nucleotide exchange factor 5 (Guanine nucleotide exchange factor for Rap1) (M-Ras-regulated Rap GEF) (MR-GEF),FUNCTION: Guanine nucleotide exchange factor (GEF) for RAP1A, RAP2A and MRAS/M-Ras-GTP. Its association with MRAS inhibits Rap1 activation (By similarity). {ECO:0000250}.

Molecular Weight: 93.7 kDa

UniProt: Q8C0Q9

AlphaFold: Q8C0Q9

Pathways: RTK Signaling, Neurotrophin Signaling Pathway, Hepatitis C