DIO3 Protein (AA 1-304) (His tag)

Catalog No. ABIN7560148

Product Details

Target: DIO3 (Deiodinase, Iodothyronine, Type III (DIO3))

Protein Type: Recombinant

Protein Characteristics: AA 1-304

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This DIO3 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Dio3 Protein expressed in mammalian cells.

Sequence: MPRQAASRLV VGEGEGPPGA SGPAATMLRS LLLHSLRLCA QTASCLVLFP RFLGTAFMLW LLDFLCIRKH FLRRRHPDHP EPEVELNSEG EEMPPDDPPI CVSDDNRLCT LASLKAVWHG QKLDFFKQAH EGGPAPNSEV VRPDGFQSQR ILDYAQGTRP LVLNFGSCTU PPFMARMSAF QRLVTKYQRD VDFLIIYIEE AHPSDGWVTT DSPYVIPQHR SLEDRVSAAR VLQQGAPGCA LVLDTMANSS SSAYGAYFER LYVIQSGTIM YQGGRGPDGY QVSELRTWLE RYDEQLHGTR PHRF Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: DIO3 (Deiodinase, Iodothyronine, Type III (DIO3))

Alternative Name: Dio3 (DIO3 Products)

Background: Thyroxine 5-deiodinase (EC 1.21.99.3) (5DIII) (DIOIII) (Type 3 DI) (Type III iodothyronine deiodinase),FUNCTION: Responsible for the deiodination of T4 (3,5,3',5'-tetraiodothyronine) into RT3 (3,3',5'-triiodothyronine) and of T3 (3,5,3'-triiodothyronine) into T2 (3,3'-diiodothyronine). RT3 and T2 are inactive metabolites. May play a role in preventing premature exposure of developing fetal tissues to adult levels of thyroid hormones. Can regulate circulating fetal thyroid hormone concentrations throughout gestation. Essential role for regulation of thyroid hormone inactivation during embryological development. {ECO:0000250|UniProtKB:P49897}.

Molecular Weight: 34.1 kDa

UniProt: Q91ZI8

AlphaFold: Q91ZI8

Pathways: Hormone Activity