LIN54 Protein (AA 1-749) (His tag)

Catalog No. ABIN7563794

This Recombinant LIN54 protein is expressed in HEK-293 Cells.

Quick Overview for LIN54 Protein (AA 1-749) (His tag) (ABIN7563794)

Target: LIN54 (Lin-54 Homolog (LIN54))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-749

Purification tag / Conjugate: This LIN54 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Lin54 Protein expressed in mammalian cells.

Sequence: MEVVPAEVNS LLPDDIMDTA ITLVDEDSIE AVIVSSPIPM ETELEEIVNI NSTGDSTATP ISTEPITVYS NHTNQVAVNT TVSKADSNTT VKPAFPSGLQ KLGAQTPVTI SANQIILNKV SQTSDLKLGN QTLKPDGQKL ILTTLGKSGS PIVLALPHSQ LPQAQKVTAQ AQPGDAKLPP QQIKVVTIGG RPEVKPVIGV SALTPGSQLI NTTTQPSVLQ TQQLKTVQIA KKPRTPTSGP VITKLIFAKP INSKAVTGQT TQASPPVVTG RVLSQSTPGT PSKTITISES GVIGSTLNST TQTPNKIAIS PLKSPNKTVK SAVQTITVGG MSTSQFKTII PLATAPNVQQ IQVPGSKFHY VRLVTATTAS SSAQPVSQSP SVNTQPLQQA KPVVVNTTPV RMSVPFVQAQ AVKQVVPKPI NSTSQIVTTS QPQQRLIMPA TPLPQIQPNL TNLPPGTVLA PAPGTGNVGY AVLPAQYVTQ LQQSSYVSIA SNSNFTGTSG IQTQARLPFN GIIPSESTSR PRKPCNCTKS LCLKLYCDCF ANGEFCNNCN CTNCYNNLEH ENERQKAIKA CLDRNPEAFK PKIGKGKEGE SDRRHSKGCN CKRSGCLKNY CECYEAKIMC SSICKCIGCK NFEESPERKT LMHLADAAEV RVQQQTAAKT KLSSQISDLL TRPTPALNSA GGKLPFTFVT KEVAEATCNC LLAQAEQADK KGKSKAAAER MILEEFGRCL MSVINSAGKA KSDPCAMHC Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: LIN54 (Lin-54 Homolog (LIN54))

Alternative Name: Lin54

Background: Protein lin-54 homolog,FUNCTION: Component of the DREAM complex, a multiprotein complex that can both act as a transcription activator or repressor depending on the context. In G0 phase, the complex binds to more than 800 promoters and is required for repression of E2F target genes. In S phase, the complex selectively binds to the promoters of G2/M genes whose products are required for mitosis and participates in their cell cycle dependent activation. In the complex, acts as a DNA-binding protein that binds the promoter of CDK1 in a sequence-specific manner. Specifically recognizes the consensus motif 5'-TTYRAA-3' in target DNA. {ECO:0000250|UniProtKB:Q6MZP7}.

Molecular Weight: 79.6 kDa

UniProt: Q571G4

AlphaFold: Q571G4

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases