POT1 Protein (AA 1-640) (His tag)

Catalog No. ABIN7564795

This Recombinant POT1 protein is produced in HEK-293 Cells.

Quick Overview for POT1 Protein (AA 1-640) (His tag) (ABIN7564795)

Target: POT1 (Protection of Telomeres 1 (POT1))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purity: > 90 % as determined by Bis-Tris PAGE, anti-tag ELISA, Western Blot and analytical SEC (HPLC)

Grade: custom-made

Product Details

Protein Characteristics: AA 1-640

Purification tag / Conjugate: This POT1 protein is labelled with His tag.

Purpose: Predefined custom protein recombinant Pot1 Protein expressed in mammalian cells.

Sequence: MSLVSTAPYT YTPLNLLKEG TIANVYGVVK FFKPPYVSKG TDYCSVVTIV DQTNVKLTCM LFSGNYEALP IIYKVGDIVR FHRLKIQVYK NELQGINCSG FASLTFEGTV GMPVTARTSS KVFSFTPQDQ KMVEALRVWA SKHISASSTL VQLCDAQPMQ YYDLTCQLLG KAQVDSTAFL LKVWDGTQTV LPSWRVSTQD LTFEGDLSHI ERLQSLVVDI LVYDNHVQVA RSIEVGCFLR LYSLHTKLQP GNSETSSSES LRLEFHLHGG TSYGRGIRVL PDTSPCVDQL KKALEGANLP VTETSTGICQ SENGDSSALS NSGSGAVSPY EEERCQQVSA TILTNHQHLE KTPLCAILTQ KAPQQYRVRA KLRSYLPRRL SQSVKLLCPK CHSVQEVPHG DSLDKILQDA ATEAPDIKLK ATSLYYSKVW TTEDQGGRQV AVHFVKNNGI LPASSECLIL IEGGRLCEVS KLSSKFHSVM PVRSGPESLE LLTLSAPFLI QGKVHHYGCK QCSSLKPIQN LNSRFHKGPW TPSSVAEALG VVPLQYVFVM VFTLDDGTGV LEAYLKDSEH FFKIPASEVL TDDDLQRSLE TIMDMICPPG IKVDAYPWLE CLLKSYNVTI GTERRICYQI FDTTVAENVV Sequence without tag. The proposed Strep-Tag is based on experience with the expression system. Our team may suggest an additional tag depending on the complexity of the protein. If you have a special request, please contact us.

Specificity: If you are looking for a specific domain and are interested in a partial protein or a different isoform, please contact us regarding an individual offer.

Characteristics: Key Benefits:

• Predefined custom protein - from design to production - by highly experienced protein experts.
• Protein expressed in mammalian cells and purified in one-step affinity chromatography
• The optimized expression system ensures reliability for intracellular, secreted and transmembrane proteins.
• State-of-the-art algorithm used for plasmid design (Gene synthesis).

This protein is a predefined protein and will be made for the first time for your order. Our experts in the lab try to ensure that you receive soluble protein.

If you are not interested in a full length protein, please contact us for individual protein fragments.

The big advantage of ordering our predefined custom proteins in comparison to ordering custom-made proteins from other companies is that there is no financial obligation in case the protein cannot be expressed or purified.

Application Details

Application Notes: We expect the protein to work for functional studies. As the protein has not been tested for functional studies yet we cannot offer a guarantee though.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: The buffer composition is at the discretion of the manufacturer.

Handling Advice: Avoid repeated freeze-thaw cycles.

Storage: -80 °C

Storage Comment: Store at -80°C.

Expiry Date: 12 months

Target Details

Target: POT1 (Protection of Telomeres 1 (POT1))

Alternative Name: Pot1

Background: Protection of telomeres protein 1 (mPot1) (POT1-like telomere end-binding protein),FUNCTION: Component of the telomerase ribonucleoprotein (RNP) complex that is essential for the replication of chromosome termini. Is a component of the double-stranded telomeric DNA-binding TRF1 complex which is involved in the regulation of telomere length by cis-inhibition of telomerase. Also acts as a single-stranded telomeric DNA-binding protein and thus may act as a downstream effector of the TRF1 complex and may transduce information about telomere maintenance and/or length to the telomere terminus. Component of the shelterin complex (telosome) that is involved in the regulation of telomere length and protection. Shelterin associates with arrays of double-stranded TTAGGG repeats added by telomerase and protects chromosome ends, without its protective activity, telomeres are no longer hidden from the DNA damage surveillance and chromosome ends are inappropriately processed by DNA repair pathways. Binds to two or more telomeric single-stranded 5'-TTAGGG-3' repeats (G-strand) and with high specificity to a minimal telomeric single-stranded 5'-TAGGGTTAG-3' sequence. Binds telomeric single-stranded sequences internally or at proximity of a 3'-end. Its activity is TERT dependent but it does not increase TERT activity (By similarity). {ECO:0000250}.

Molecular Weight: 70.9 kDa

UniProt: Q91WC1

AlphaFold: Q91WC1

Pathways: Cell Division Cycle, Telomere Maintenance