CTLA4 Protein (AA 36-161)

Catalog No. ABIN7566055

Recombinant CTLA4 protein expressed in HEK-293 Cells.

Quick Overview for CTLA4 Protein (AA 36-161) (ABIN7566055)

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: >95 % (SDS-PAGE)

Product Details

Protein Characteristics: AA 36-161

Purpose: CD152 [CTLA-4] (human) (rec.) (untagged)

Cross-Reactivity: Human

Characteristics: Human CD152 [CTLA-4] (aa 36-161) is untagged.

Endotoxin Level: <0.01EU/μg purified protein (LAL test).

Biological Activity Comment: Binds to human CD80.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: 1 mg/mL after reconstitution.

Concentration: 1 mg/mL

Buffer: Contains PBS.

Handling Advice: After opening, prepare aliquots and store at -20 °C. Avoid freeze/thaw cycles. Centrifuge lyophilized vial before opening and reconstitution.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 6 months after receipt when stored at -20°C.Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: CTLA4 (Cytotoxic T-Lymphocyte-Associated Protein 4 (CTLA4))

Alternative Name: CD152 [CTLA-4]

Background:

Cytotoxic T-lymphocyte-associated Antigen 4, Cytotoxic T-lymphocyte Protein 4

CD152 (Cytotoxic T-lymphocyte-associated protein 4, CTLA-4) is an inhibitory receptor belonging to the CD28 immunoglobulin subfamily, expressed primarily by T cells. Its ligands, CD80 and CD86, are typically found on the surface of antigen-presenting cells and can either bind CD28 or CTLA-4, resulting in a costimulatory or a co-inhibitory response, respectively. Because of its dampening effect, CTLA-4 is a crucial regulator of T cell homeostasis and self-tolerance. While CD28 promotes T cell activation and proliferation, CTLA-4 is reported to dampen T cell responses through a variety of mechanisms. Prior to activation, conventional T cells (Tconv) express low levels of CTLA-4, predominantly in intracellular compartments. Upon activation, CTLA-4 expression is upregulated and becomes increasingly detectable on the cell surface. In Tregs on the other hand, transmembrane CTLA-4 is constitutively expressed and plays an important role in Treg homeostasis and function. In general, T cell CTLA-4 is largely constrained to intracellular expression although some surface expression may be detectable owing to the rapid, continuous shuttling of CTLA-4 between intracellular compartments and the plasma membrane. Recently, CTLA-4 has been studied in dendritic cells and tumors, showing that CTLA-4 plays nonredundant and critical roles in thymic development, T cell priming, peripheral tolerance, and a variety of other critical immunoregulatory functions as an immune checkpoint in Immuno-oncology research.

Molecular Weight: ~18kDa (SDS-PAGE)

UniProt: P16410

Pathways: Cancer Immune Checkpoints