IL12 Protein (AA 23-328, AA 57-253) (His tag)

Catalog No. ABIN7566183

Product Details

Target: IL12 (Interleukin 12 (IL12))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 23-328, AA 57-253

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IL12 protein is labelled with His tag.

Purpose: IL-12 (human) (rec.) (His)

Cross-Reactivity: Human

Characteristics: The human IL-12 complex composed of the p40 subunit (aa 23-328) and the p35 subunit (aa 57-253) is fused at the C-terminus through a (G3S)4 linker to a His-tag.

Purity: >95 % (SDS-PAGE)

Sterility: Sterile filtered

Endotoxin Level: <0.01EU/μg protein (LAL test, Lonza).

Biological Activity Comment: The ED50 was determined by the induction of IFN-gamma from NK cells co-stimulated with IL-18 is < 1.0 ng/ml, corresponding to a specific activity of >1 x 106units/mg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from 0.2μm-filtered solution in PBS.

Handling Advice: Avoid freeze/thaw cycles.Centrifuge lyophilized vial before opening and reconstitution.PBS containing at least 0.1 % BSA should be used for further dilutions.

Storage: 4 °C,-20 °C

Storage Comment:

Short Term Storage: +4°C

Long Term Storage: -20°C

Use & Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Target Details

Target: IL12 (Interleukin 12 (IL12))

Alternative Name: IL-12 (IL12 Products)

Background:

Interleukin-12, IL-12p70, IL-12p75, Cytotoxic Lymphocyte Maturation Factor, CLMF, NKSF, Natural Killer Cell Stimulatory Factor, TSF, T-cell Stimulatory Factor

Interleukin-12 (IL-12), also known as Natural Killer Cell Stimulatory Factor (NKSF) or Cytotoxic Lymphocyte Maturation Factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. IL-12 is produced by macrophages and B lymphocytes and has been shown to have multiple effects on T cells and Natural Killer (NK) cells. Some of these IL-12 activities include the induction of IFN-gamma and TNF in resting and activated T and NK cells, the enhancement of cytotoxic activity of resting NK and T cells, the stimulation of resting T cell proliferation in the presence of a comitogen, and the enhancement of NK cell proliferation. Current evidence indicates that IL-12 is a key mediator of cellular immunity and induces the differentiation of Th1 cells from precursor T helper cells. Based on its activities, it has been suggested that IL-12 may have therapeutic potential as a vaccine adjuvant that promotes cellular immunity and as an antitumor and anti viral agent.

Molecular Weight: ~85kDa (SDS-PAGE)

NCBI Accession: NP_000873

Pathways: JAK-STAT Signaling, TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome