GDNF Protein (Biotin)

Catalog No. ABIN7582099

Recombinant GDNF protein expressed in Escherichia coli (E. coli).

Quick Overview for GDNF Protein (Biotin) (ABIN7582099)

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), Cell Culture (CC), Flow Cytometry (FACS), Live Cell Imaging (LCI), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details

Purification tag / Conjugate: This GDNF protein is labelled with Biotin.

Purpose: human GDNF-Biotin

Sequence: SPDKQMAVLP RRERNRQAAA ANPENSRGKG RRGQRGKNRG CVLTAIHLNV TDLGLGYETK EELIFRYCSG SCDAAETTYD KILKNLSRNR RLVSDKVGQA CCRPIAFDDD LSFLDDNLVY HILRKHSAKR CGCI-OH

Specificity: Toxin target: GFRA1 (GDNF Receptor alpha 1), RET Receptor Tyrosine Kinase

Characteristics: LC-Biotin. The extent of labeling is 1-2 Molecules of biotin per one recombinant human GDNF protein, linked via primary amines.

Biological Activity Comment: GDNF enhances survival and differentiation of dopaminergic neurons. It is also a potent survival factor for motor neurons1-3.

Application Details

Application Notes: Antigen Preadsorption Control: 1 nM - 0.1 μM

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial and dissolve by gently tapping, tilting, or rolling the vial. Avoid vigorous vortexing, a brief, light vortex of 1-2 seconds is acceptable if necessary. The product is readily soluble in high-quality pure water at micromolar to millimolar concentrations (typically 5 μM to 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x the final working concentration. Divide the stock solution into small, single-use aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh working solutions just before use. Avoid multiple freeze-thaw cycles to maintain optimal biological activity.

Buffer: Lyophilized from double distilled water (ddH2O). May contain residual trifluoroacetate (TFA).

Handling Advice: Centrifuge the vial at 10,000 x g for 5 minutes before opening to ensure all material is collected at the bottom,Avoid multiple freeze-thaw cycles to maintain optimal biological activity,Avoid vigorous vortexing. protect from moisture

Storage: -20 °C

Storage Comment: The product is shipped as a lyophilized powder at room temperature. Upon receipt, store at -20°C and protect from moisture.

Target Details

Target: GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))

Alternative Name: GDNF

Background: A biotinylated human GDNF for imaging and sensitive detection of GFRα1/RET receptors and the study of dopaminergic neuron survival

Molecular Weight: 30.8 kDa (dimer)

Pathways: RTK Signaling, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration