IL-21 Protein (AA 18-146)

Catalog No. ABIN7596304

This Recombinant IL-21 protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-21 Protein (AA 18-146) (ABIN7596304)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Protein Type: Recombinant

Biological Activity: Active

Origin: Dog

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Activity Assay (AcA)

Purity: > 95% by SDS-PAGE

Product Details

Protein Characteristics: AA 18-146

Sequence: MHKSSFQEQD LLLIRMRQLI DIVDQLKNYV NDLDPESLPA PEDVKRHCER SAFSCFQKVQ LKAANTGGNE QIINVLTKQL KRKLPPTNAG RRQKHRPACP SCDSYEKAPP KEFLERLKSL IQKMIHQHLS

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: The activity is determined by the IFN-g ELISA in a using NK-92 human natural killer cells. The ED50 range ≤ 2 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL-21

Background: IL-21, also known as Interleukin-21, is an important modulator of natural killer (NK) cell function. However, little is known about IL-21 function in canine NK cells because the phenotype of these cells remains undefined. NK cells proliferated rapidly in response to activation by IL-21 for 3 weeks, and IL-21 was able to induce changes in the mRNA expression of NK cell-related receptors and enhance the effector function of NK cells in perforin- and granzyme-B-dependent manners. The duration, frequency and timing of IL-21 stimulation during culture affected the rate of proliferation, patterns of receptor expression, cytokine production, and anti-tumor activity. It has a potential predicating that synergistic interactions of IL-21 with IL-2 and IL-15 play an important role in the proliferation, receptor expression, and effector function of canine NK cells. Recombinant canine IL-21, was expressed in E. coli and purified by using conventional chromatography techniques.

Molecular Weight: 15 kDa (130aa), confirmed by MALDI-TOF

NCBI Accession: NP_001003347

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response