IL-21 Protein (AA 30-162)

Catalog No. ABIN7596307

This Recombinant IL-21 protein is produced in Escherichia coli (E. coli).

Quick Overview for IL-21 Protein (AA 30-162) (ABIN7596307)

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: SDS-PAGE (SDS), Activity Assay (AcA)

Purity: > 95% by SDS - PAGE

Grade: Animal-Free

Product Details

Protein Characteristics: AA 30-162

Sequence: MQGQDRHMIR MRQLIDIVDQ LKNYVNDLVP EFLPAPEDVE TNCEWSAFSC FQKAQLKSAN GNNERIINV SIKKLKRKPP STNAGRRQKH RLTCPSCDSY EKKPPKEFLE RFKSLLQKMI HQHLSSRTHG SEDS

Endotoxin Level: < 1 EU per 1ug of protein (determined by LAL method)

Biological Activity Comment: The activity is determined by the IFN-γ ELISA in a using NK-92 human natural killer cells. The ED50 range ≤ 4 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Can be stored at +2°C to +8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.

Target Details

Target: IL-21 (IL21) (Interleukin 21 (IL21))

Alternative Name: IL-21 Protein

Background: IL-21, also known as Interleukin-21, is a T-helper cytokine that regulates humoral immunity and cell-mediated anti-tumor responses. It has broad immunoregulatory activity and can modulate both humoral and cell-mediated responses. It is important in the regulation of haematopoiesis and immune responses, and can influence lymphocyte development. It is produced by activated T cell, and it influences proliferation of T and B cells and cytolytic activity of natural killer cells. It may be a critical factor in the control of persistent viral infections. It has a potential predicating significance for survival Time in patients in the development of sporadic CRC. It binds to the IL-21R and can activate janus kinase JAK1, JAK3, STAT1, and STAT3 in EDgamma-16 cells. Recombinant human IL-21, was expressed in E. coli and purified by using conventional chromatography techniques. Produced using non-animal reagents in an animal-free laboratory

Molecular Weight: 15.5 kDa (134aa) confirmed by MALDI-TOF

NCBI Accession: NP_068575

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response