USH2A Protein (AA 3956-4020) (Fc Tag)

Catalog No. ABIN7853222

Recombinant USH2A protein expressed in HEK-293 Cells.

Quick Overview for USH2A Protein (AA 3956-4020) (Fc Tag) (ABIN7853222)

Target: USH2A (Usher Syndrome 2A (Autosomal Recessive, Mild) (USH2A))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 3956-4020

Purification tag / Conjugate: This USH2A protein is labelled with Fc Tag.

Purpose: Recombinant human USH2A Protein with C-terminal human Fc tag

Specificity: USH2A(Ser3956-Thr4020) hFc(Glu99-Ala330)

Characteristics: Extracellular Domain Protein

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: USH2A (Usher Syndrome 2A (Autosomal Recessive, Mild) (USH2A))

Alternative Name: USH2A

Background: US2, RP39, USH2, dJ1111A8.1
This gene encodes a protein that contains laminin EGF motifs, a pentaxin domain, and many fibronectin type III motifs. The protein is found in the basement membrane, and may be important in development and homeostasis of the inner ear and retina. Mutations within this gene have been associated with Usher syndrome type IIa and retinitis pigmentosa. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2008]

Molecular Weight: predicted molecular mass of 33.4 kDa after removal of the signal peptide. The apparent molecular mass of USH2A-hFc is 35-70 kDa due to glycosylation.

UniProt: O75445

Pathways: Sensory Perception of Sound