ZNRF3 Protein (AA 99-330) (Fc Tag)

Catalog No. ABIN7852976

Recombinant ZNRF3 protein expressed in HEK-293 Cells.

Quick Overview for ZNRF3 Protein (AA 99-330) (Fc Tag) (ABIN7852976)

Target: ZNRF3 (Zinc and Ring Finger Protein 3 (ZNRF3))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Application: Affinity Measurement (AM), Antibody Drug Assay (ADA), Immunoassay (IA), Screening Assay (ScA)

Purity: The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.

Product Details

Protein Characteristics: AA 99-330

Purification tag / Conjugate: This ZNRF3 protein is labelled with Fc Tag.

Purpose: Recombinant human ZNRF3 Protein with N-terminal human Fc tag

Specificity: HFc(Glu99-Ala330) ZNRF3(Lys56-Met219)

Characteristics: Extracellular Domain Protein

Application Details

Application Notes: Extracellular Domain Proteins (ECD) can be used as:

• Immunogens for antibody drug development
• Reagents used for CAR-T positive cell monitoring
• Reagents for antibody screening and functional testing
• Reagents for antibody affinity measurement

Comment:

The protein was produced using HEK293 mammalian cell secretion expression system to ensure the close-to-native structures and post-translational modifications of the target protein.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please see Certificate of Analysis for specific instructions of reconstitution.

Buffer: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose is added as protectants before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: Store at -20°C to -80°C for 12 months in lyophilized form. After reconstitution, if not intended for use within a month, aliquot and store at -80°C (Avoid repeated freezing and thawing). Lyophilized proteins are shipped at ambient temperature.

Expiry Date: 12 months

Target Details

Target: ZNRF3 (Zinc and Ring Finger Protein 3 (ZNRF3))

Alternative Name: ZNRF3

Background: RNF203, BK747E2.3
Enables frizzled binding activity and ubiquitin-protein transferase activity. Involved in cellular protein metabolic process and negative regulation of Wnt signaling pathway. Is integral component of plasma membrane. [provided by Alliance of Genome Resources, Apr 2022]

Molecular Weight: predicted molecular mass of 44.3 kDa after removal of the signal peptide. The apparent molecular mass of hFc-ZNRF3 is 35-55 kDa due to glycosylation.

UniProt: Q9ULT6

Pathways: WNT Signaling