HMGB1 Protein (AA 1-215) (His tag)

Catalog No. ABIN7607153

This Recombinant HMGB1 protein is expressed in Mammalian Cells.

Quick Overview for HMGB1 Protein (AA 1-215) (His tag) (ABIN7607153)

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Immunogen (Imm), Activity Assay (AcA)

Purity: >95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-215

Purification tag / Conjugate: This HMGB1 protein is labelled with His tag.

Purpose: Recombinant Human HMGB1 Protein, C-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized human HMGB1 (Cat #: AHC40301) at 2μg/mL (100 μL/well) can bind human AGER/RAGE with a linear range of 0.1-42.8 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: HMGB1 (High Mobility Group Box 1 (HMGB1))

Alternative Name: High mobility group protein B1

Background: High mobility group protein B1, HMGB1, HMG1, HMG-1, High mobility group protein 1

UniProt: P09429

Pathways: p53 Signaling, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Toll-Like Receptors Cascades, Smooth Muscle Cell Migration, Inflammasome