IGFBP5 Protein (AA 21-272) (His tag)

Catalog No. ABIN7678449

This Recombinant IGFBP5 protein is produced in Mammalian Cells.

Quick Overview for IGFBP5 Protein (AA 21-272) (His tag) (ABIN7678449)

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), Immunogen (Imm), ELISA, Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 21-272

Purification tag / Conjugate: This IGFBP5 protein is labelled with His tag.

Purpose: Recombinant Human IGFBP5 Protein, C-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on human breast cancer cells (MCF-7). The ED50 for this effect is 0.5-1.5 μg/mL in the presence of 14 ng/mL rhIGF-II.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Alternative Name: IGF-binding protein 5

Background: IGF-binding protein 5, IGFBP5, Insulin-like growth factor-binding protein 5, IGFBP-5, IBP-5, IBP5

UniProt: P24593

Pathways: WNT Signaling, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding