EPH Receptor A3 Protein (EPHA3) (AA 1-541) (His tag)

Catalog No. ABIN7678472

Recombinant EPH Receptor A3 protein expressed in Mammalian Cells.

Quick Overview for EPH Receptor A3 Protein (EPHA3) (AA 1-541) (His tag) (ABIN7678472)

Target: EPH Receptor A3 (EPHA3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: SDS-PAGE (SDS), Western Blotting (WB), ELISA, Activity Assay (AcA), Immunogen (Imm)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 1-541

Purification tag / Conjugate: This EPH Receptor A3 protein is labelled with His tag.

Purpose: Recombinant Human EPHA3 Protein, C-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. When recombinant human EphA3 (ABIN7607412 and ABIN7678472) is coated at 2 μg/mL (100 μL/well), the concentration of biotinylayed rhEphrin A5 that produces 50% of the optimal binding response is found to be approximately 5-25 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: EPH Receptor A3 (EPHA3)

Alternative Name: HEK

Background: HEK, Eph-like tyrosine kinase 1, EK4, EPHA3, Tyrosine-protein kinase receptor ETK1, Tyrosine-protein kinase TYRO4, Ephrin type-A receptor 3, hEK4, ETK, TYRO4, ETK1, Human embryo kinase, EPH-like kinase 4

UniProt: P29320

Pathways: RTK Signaling, Regulation of Cell Size