SEMA3B Protein (AA 25-727, R551A, R554A) (His-Fc tag)

Catalog No. ABIN7678595

Recombinant SEMA3B protein expressed in Mammalian Cells.

Quick Overview for SEMA3B Protein (AA 25-727, R551A, R554A) (His-Fc tag) (ABIN7678595)

Target: SEMA3B (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), Immunogen (Imm), ELISA, Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 25-727, R551A, R554A

Purification tag / Conjugate: This SEMA3B protein is labelled with His-Fc tag.

Purpose: Recombinant Human SEMA3B Protein, C-hFc-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. When recombinant human SEMA3B (ABIN7607544 and ABIN7678595) is coated at 1 μg/mL, 100 μL/well, it binds to recombinant Rat Neuropilin-1 with an ED50 of 25-150 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: SEMA3B (Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3B (SEMA3B))

Alternative Name: Semaphorin-3B

Background: Semaphorin-3B, SEMA5, Sema A(V), SEMAA, Semaphorin-V, SEMA3B, Sema V

UniProt: Q13214