RGMA Protein (AA 48-422) (His tag)

Catalog No. ABIN7678724

This Recombinant RGMA protein is produced in Mammalian Cells.

Quick Overview for RGMA Protein (AA 48-422) (His tag) (ABIN7678724)

Target: RGMA (RGM Domain Family, Member A (RGMA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: ELISA, Western Blotting (WB), SDS-PAGE (SDS), Immunogen (Imm), Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 48-422

Purification tag / Conjugate: This RGMA protein is labelled with His tag.

Purpose: Recombinant Human RGMA Protein, C-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. When rhBMP-4 is coated at 1 μg/mL, rhRGMA (ABIN7607677 and ABIN7678724) binds with an apparent KD <50nM. Immobilized rhRGMA (ABIN7607677 and ABIN7678724) can bind rmNeogenin/Fc Chimera with a linear range of 10-500 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: RGMA (RGM Domain Family, Member A (RGMA))

Alternative Name: RGM domain family member A

Background: RGM domain family member A, RGMA, RGM, Repulsive guidance molecule A

UniProt: Q96B86

Pathways: Tube Formation