R-Spondin 3 Protein (RSPO3) (AA 22-272) (His-Fc tag)

Catalog No. ABIN7678744

Recombinant R-Spondin 3 protein expressed in Mammalian Cells.

Quick Overview for R-Spondin 3 Protein (RSPO3) (AA 22-272) (His-Fc tag) (ABIN7678744)

Target: R-Spondin 3 (RSPO3)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Mammalian Cells

Application: Western Blotting (WB), SDS-PAGE (SDS), ELISA, Immunogen (Imm), Activity Assay (AcA)

Purity: >90 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 22-272

Purification tag / Conjugate: This R-Spondin 3 protein is labelled with His-Fc tag.

Purpose: Recombinant Human RSPO3 Protein, C-hFc-His (Active)

Endotoxin Level: <0.1 EU/μg of the protein by the LAL method.

Biological Activity Comment: Measured by its ability to induce Topflash reporter activity in human embryonic kidney cells (HEK293T). The ED50 for this effect is 0.50-2.00 ng/mL in the presence of 5 ng/mL recombinant Mouse Wnt-3a.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a solution in PBS pH 7.4, 5 % Trehalose, 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for one week. Store at -20 to -80°C for twelve months from the date of receipt.

Expiry Date: 12 months

Target Details

Target: R-Spondin 3 (RSPO3)

Alternative Name: RSPO3

Background: RSPO3, hPWTSR, hRspo3, PWTSR, R-spondin-3, Thrombospondin type-1 domain-containing protein 2, Protein with TSP type-1 repeat, Roof plate-specific spondin-3, THSD2

UniProt: Q9BXY4