BDNF Protein (AA 129-247)

Catalog No. ABIN7662894

This Recombinant BDNF protein is produced in Escherichia coli (E. coli).

Quick Overview for BDNF Protein (AA 129-247) (ABIN7662894)

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Protein Type: Recombinant

Origin: Human, Rat, Mouse

Source: Escherichia coli (E. coli)

Application: Western Blotting (WB), ELISA

Purity: Greater than 95 % as determined by SDS-PAGE.

Product Details

Protein Characteristics: AA 129-247

Purpose: Recombinant Human/Murine/Rat BDNF

Endotoxin Level: < 0.01 EU/μg as determined by LAL test.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial at 10000 rpm for 30 s before opening, reconstitute in sterile distilled water or buffer of interest to concentration of 0.1-1 mg/mL by gently pipetting 2-3 times, don't vortex.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.2.

Storage: -20 °C,-80 °C

Storage Comment: The lyophilized protein is stable at -20 °C for up to 1 year. After reconstitution, the protein solution is stable at -20 to -80 °C for 3 months or 1 week at 2 to 8 °C under sterile conditions. For extended storage, it is recommended to further dilute in working aliquots, avoid repeated freeze/thaw cycle.

Expiry Date: 12 months

Target Details

Target: BDNF (Brain-Derived Neurotrophic Factor (BDNF))

Alternative Name: BDNF

Background: Brain-Derived Neurotrophic Factor, BDNF, Abrineurin

Molecular Weight: 14 kDa

UniProt: P23560

Pathways: RTK Signaling, Synaptic Membrane, Feeding Behaviour, Dicarboxylic Acid Transport, Regulation of long-term Neuronal Synaptic Plasticity