ACP5 Protein (AA 1-327) (His tag)

Catalog No. ABIN7668093

Recombinant ACP5 protein expressed in Mammalian Cells.

Quick Overview for ACP5 Protein (AA 1-327) (His tag) (ABIN7668093)

Target: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Mouse

Source: Mammalian Cells

Purity: > 95 %

Product Details

Protein Characteristics: AA 1-327

Purification tag / Conjugate: This ACP5 protein is labelled with His tag.

Purpose: Recombinant Mouse Acp5 Protein(His Tag)

Sequence: Met1-Pro327

Purification: SDS-PAGE analysis of Mouse Acp5 proteins, 2μg/lane of Recombinant Mouse Acp5 proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 36 kDa

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU/mg of the protein as determined by the LAL method

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: ACP5 (Acid Phosphatase 5, Tartrate Resistant (ACP5))

Alternative Name: Acp5

Background: Tartrate-resistant acid phosphatase (TRACP) or acid phosphatase 5, tartrate resistant (ACP5 or TRAP) is a glycosylated monomeric metalloenzyme expressed in mammals. TRACP is associated with osteoblast migration to bone resorption sites, and, once there, TRACP is believed to initiate osteoblast differentiation, activation, and proliferation. TRACP once considered to be just a histochemical marker of osteoclasts is now recognised to be a molecule of widespread occurrence with functions in both the skeleton and the immune system. Two forms of TRACP circulate in human blood, TRACP 5a derived from macrophages and dendritic cells, and TRACP-5b derived from osteoclasts. Recent data have demonstrated the utility of TRACP-5b as a marker of osteoclast number and bone resorption, and serum TRACP-5a as a marker of inflammatory conditions. TRACP is expressed by osteoclasts, macrophages, dendritic cells and a number of other cell types. It has a critical role in many biological processes including skeletal development, collagen synthesis and degradation, the mineralisation of bone, cytokine production by macrophages and dendritic cells, macrophage recruitment, dendritic cell maturation and a role in the development of Th1 responses.,ACP,PPA,Tartrate-resistant acid phosphatase type,EC 3.1.3.2,TR-AP,TrATPase,Acp5,T5ap,Trap,PPA5,Tartrate-resistant acid phosphatase type 5,Tartrate-resistant acid ATPase,Type 5 acid phosphatase,SPENCDI,TRACP,TRAP5b

Molecular Weight:

calculated_mw: 35.9 kDa

observed_mw: 36 kDa

UniProt: Q05117

Pathways: Transition Metal Ion Homeostasis