ADGRE5 (AA 21-530) protein (Fc Tag)

Catalog No. ABIN7668119

This Recombinant ADGRE5 protein is expressed in Mammalian Cells.

Quick Overview for ADGRE5 (AA 21-530) protein (Fc Tag) (ABIN7668119)

Target: ADGRE5

Protein Type: Recombinant

Origin: Human

Source: Mammalian Cells

Application: Immunogen (Imm)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 21-530

Purification tag / Conjugate: Fc Tag

Purpose: Recombinant Human ADGRE5 Protein( Fc Tag)

Sequence: Gln21-Leu530

Purification: SDS-PAGE analysis of Human ADGRE5 proteins, 2 μg/lane of Recombinant Human ADGRE5 proteins was resolved with SDS-PAGE under reducing conditions, showing bands at 80.9 KD

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: ADGRE5

Background: ADGRE5,Adhesion G protein-coupled receptor E5,CD 97,CD_antigen=CD97,CD97,CD97 antigen,CD97 antigen subunit beta,CD97 Molecule,CD97,Leukocyte antigen CD97,Seven span transmembrane protein,Seven transmembrane helix receptor,Seven transmembrane heterodimeric receptor associated with inflammation,TM7LN 1,TM7LN1,The cluster of differentiation (CD) system is commonly used as cell markers in immunophenotyping. Different kinds of cells in the immune system can be identified through the surface CD molecules which associating with the immune function of the cell. There are more than 32 CD unique clusters and subclusters have been identified. Some of the CD molecules serve as receptors or ligands important to the cell through initiating a signal cascade which then alter the behavior of the cell. Some CD proteins do not take part in cell signal process but have other functions such as cell adhesion. The CD97 is a receptor predominantly expressed in leukocytes and belongs to a new group of seven-span transmembrane molecules, which is also designed EGF-TM7 family. The family members are characterized by an extended extracellular region with several N-terminal epidermal growth factor-like domains two of which contain a calcium-binding site. Mature CD 97 has two noncovalently associated subunits and is composed of a large extracellular protein (CD97 alpha) and a seven-membrane spanning protein (CD97 beta). CD97 is considered as a defining feature of G protein-coupled receptors. The effects that lymphocytes and erythrocytes adhere to CD97-transfected COS cells suggest that CD97 has the ability to bind cellular ligands. CD97 alpha has three alternatively spliced isoforms that are related to the calcium-binding EGF-like repeats in the microfibril protein fibrillin. Leukocytes strongly positive for CD97 are concentrated at sites of inflammation relative to CD97 expression in normal lymphoid tissues.

Molecular Weight:

Calculated MW: 80.9 kDa

Observed MW: 100-110 kDa

Gene ID: 976

UniProt: P48960