ADGRE5 (AA 21-530) protein (His tag)

Catalog No. ABIN7668120

Recombinant ADGRE5 protein expressed in Mammalian Cells.

Quick Overview for ADGRE5 (AA 21-530) protein (His tag) (ABIN7668120)

Target: ADGRE5

Protein Type: Recombinant

Origin: Human

Source: Mammalian Cells

Application: Immunogen (Imm)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 21-530

Purification tag / Conjugate: His tag

Purpose: Recombinant Human ADGRE5 Protein(His Tag)

Sequence: Gln21-Leu530

Purification: SDS-PAGE analysis of Human ADGRE5 proteins, 2 μg/lane of Recombinant Human ADGRE5 proteins was resolved with an SDS-PAGE under reducing conditions, showing bands at 55.9KD

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: ADGRE5

Background: ADGRE5,Adhesion G protein-coupled receptor E5,CD 97,CD_antigen=CD97,CD97,CD97 antigen,CD97 antigen subunit beta,CD97 Molecule,CD97,Leukocyte antigen CD97,Seven span transmembrane protein,Seven transmembrane helix receptor,Seven transmembrane heterodimeric receptor associated with inflammation,TM7LN 1,TM7LN1,This gene encodes a member of the EGF-TM7 subfamily of adhesion G protein-coupled receptors, which mediate cell-cell interactions. These proteins are cleaved by self-catalytic proteolysis into a large extracellular subunit and seven-span transmembrane subunit, which associate at the cell surface as a receptor complex. The encoded protein may play a role in cell adhesion as well as leukocyte recruitment, activation and migration, and contains multiple extracellular EGF-like repeats which mediate binding to chondroitin sulfate and the cell surface complement regulatory protein CD55. Expression of this gene may play a role in the progression of several types of cancer. Alternatively spliced transcript variants encoding multiple isoforms with an 3 to 5 EGF-like repeats have been observed for this gene. This gene is found in a cluster with an other EGF-TM7 genes on the short arm of chromosome 19.

Molecular Weight:

Calculated MW: 55.9 kDa

Observed MW: 80-100 kDa

Gene ID: 976

UniProt: P48960