beta-2 Microglobulin Protein (His tag)

Catalog No. ABIN7668230

This Recombinant beta-2 Microglobulin protein is expressed in HEK-293 Cells.

Quick Overview for beta-2 Microglobulin Protein (His tag) (ABIN7668230)

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 %

Product Details

Purification tag / Conjugate: This beta-2 Microglobulin protein is labelled with His tag.

Purpose: Recombinant Mouse B2M/Beta-2-microglobulin Protein (His Tag)

Sequence: Met 1-Met 119

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU/mg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: beta-2 Microglobulin (B2M) (beta-2-Microglobulin (B2M))

Alternative Name: B2M,Beta-2-microglobulin

Background: B2M, also known as β2-Microglobulin or CDABP0092, is a component of MHC class I molecules found expression in all nucleated cells (excludes red blood cells). The major function of MHC class I moleculesis is to display fragments of proteins from within the cell to T-cells and cells containing foreign proteins will be attacked. B2M (β2-Microglobulin) is a low molecular weight protein. It was demonstrated that B2M (β2-Microglobulin) was localized in the membranes of nucleated cells and was found to be associated with HL-A antigens. B2M (β2-Microglobulin) is present in free form in various body fluids and as a subunit of histocompatibility antigens on cell surfaces lateral to the α3 chain. Unlike α3, β2 has no transmembrane region. Directly above β2 lies the α1 chain, which itself is lateral to the α2. In the absence of B2M (β2 microglobulin), very limited amounts of MHC class I (classical and non-classical) molecules can be detected on the surface. In the absence of MHC class I, CD8 T cells, a subset of T cells involved in the development of acquired immunity cannot develop. Low levels of B2M (β2 microglobulin) can indicate non-progression of HIV.,B2MG,Beta 2 Microglobin,Beta 2 microglobulin Precursor,Beta Chain of MHC Class 1 Proteins,Beta Chain of MHC class I molecules,Beta-2-Microglobulin,CDABP0092,Hdcma22p,IMD43

Molecular Weight:

calculated_mw: 10.8 kDa

observed_mw: 12 kDa

UniProt: P01887

Pathways: TCR Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process