CD47 Protein (CD47) (His tag)

Catalog No. ABIN7668379

This Recombinant CD47 protein is produced in Escherichia coli (E. coli).

Quick Overview for CD47 Protein (CD47) (His tag) (ABIN7668379)

Target: CD47

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 %

Product Details

Purification tag / Conjugate: This CD47 protein is labelled with His tag.

Purpose: Recombinant Mouse CD47 protein (His tag)

Sequence: Gln 19-Lys 140

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: CD47

Alternative Name: CD47

Background: CD47, also known as Integrin-Associated Protein (IAP) and OA3, is a glycosylated atypical member of the immunoglobulin superfamily. Mouse CD47 is an integral membrane protein that consists of a extracellular domain (ECD) with a single Ig-like domain, five membrane-spanning regions with short intervening loops, and C-terminal cytoplasmic tail. CD47 has a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It plays an important role in memory formation and synaptic plasticity in the hippocampus. As a receptor for SIRPA, it binding to which prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells. Interaction with SIRPG mediates cellcell adhesion, it enhances superantigen-dependent T-cell-mediated proliferation and costimulates T-cell activation. It may play a role in membrane transport and/or integrin dependent signal transduction. It also prevents premature elimination of red blood cells.,Antigenic Surface Determinant Protein OA3,CD47,Integrin-Associated Protein,LAP,Leukocyte Surface Antigen CD47,MER6,OA3,Protein MER6

Molecular Weight:

calculated_mw: 13.3 kDa

observed_mw: 15 kDa

UniProt: Q61735