IL-18 Protein (His tag)

Catalog No. ABIN7669052

This Recombinant IL-18 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-18 Protein (His tag) (ABIN7669052)

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 %

Product Details

Purification tag / Conjugate: This IL-18 protein is labelled with His tag.

Purpose: Recombinant Human IL-18 protein (His tag)

Sequence: Tyr 37-Asp 193

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL-18 (IL18) (Interleukin 18 (IL18))

Alternative Name: IL-18

Background: Interleukin-18 (IL-18, also known as interferon-gamma inducing factor) is a proinflammatory cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. This cytokine can induce the IFN-gamma production of T cells. The combination of IL-18 and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production of B cells. IL-18 binding protein (IL18BP) can specifically interact with this cytokine, and thus negatively regulate its biological activity. IL-18 is an IL-1&minus,like cytokine that requires cleavage with caspase-1 to become active, was found to increase IgE production in a CD4+ T cells-, IL-4&minus, and STAT6&minus,dependent fashion. IL-18 and T cell receptor&minus,mediated stimulation could induce naï,ve CD4+ T cells to develop into IL-4&minus,producing cells in vitro. Thus, caspase-1 and IL-18 may be critical in regulation of IgE production in vivo, providing a potential therapeutic target for allergic disorders. IL-18 production in primary synovial cultures and purified synovial fibroblasts was, in turn, upregulated by TNF-&alpha, and IL-1&beta, suggesting that monokine expression can feed back to promote Th1 cell development in synovial membrane. Besides, synergistic combinations of IL-18, IL-12, and IL-15 may be of importance in sustaining both Th1 responses and monokine production in RA.,MGC,IL1F,IL-1F,IL18,IGIF,IL-18,IL-1g,IL1F4,GIF,Iboctadekin,IFN-gamma-inducing factor,IL-1 gamma,IL-1F4,Interferon gamma-inducing factor,Interferon-gamma-inducing factor,Interleukin 18,Interleukin-1 gamma,Interleukin-18,MGC12320,IFN gamma inducing factor,IL 1 gamma,IL 18,IL 1g,IL1 gamma,IL18 protein,IL1g,IL1gamma,ILIF4,Interferon gamma inducing factor,Interleukin 1 gamma,Interleukin 18 (interferon-gamma-inducing factor),Interleukin18

Molecular Weight:

calculated_mw: 17.2 kDa

observed_mw: 18 kDa

UniProt: Q14116

Pathways: Cellular Response to Molecule of Bacterial Origin, Activated T Cell Proliferation, Cancer Immune Checkpoints, Inflammasome