Occludin Protein (OCLN) (His tag)

Catalog No. ABIN7669390

This Recombinant Occludin protein is produced in Escherichia coli (E. coli).

Quick Overview for Occludin Protein (OCLN) (His tag) (ABIN7669390)

Target: Occludin (OCLN)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: Immunogen (Imm)

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This Occludin protein is labelled with His tag.

Purpose: Recombinant Human OCLN Protein(His Tag)

Sequence: Lys266~Thr522

Purification: SDS-PAGE analysis of Human OCLN proteins, 2 μg/lane of Recombinant Human OCLN proteins was resolved with an SDS-PAGE under reducing conditions, showing bands at 28 KD

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method

Biological Activity Comment: Not validated for activity

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with 5 % Trehalose and 5 % Mannitol.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Occludin (OCLN)

Alternative Name: OCLN

Background: BLCPMG,FLJ08163,FLJ18079,FLJ77961,FLJ94056,MGC34277,Occludin,Ocln,OCLN,Phosphatase 1 regulatory subunit 115,PPP1R115,PTORCH1,Tight junction protein occludin,This gene encodes an integral membrane protein that is required for cytokine-induced regulation of the tight junction paracellular permeability barrier. Mutations in this gene are thought to be a cause of band-like calcification with an simplified gyration and polymicrogyria (BLC-PMG), an autosomal recessive neurologic disorder that is also known as pseudo-TORCH syndrome. Alternative splicing results in multiple transcript variants. A related pseudogene is present 1.5 Mb downstream on the q arm of chromosome 5.

Molecular Weight:

Calculated MW: 28 kDa

Observed MW: 42 kDa

Gene ID: 100506658

UniProt: Q16625

Pathways: Cell-Cell Junction Organization, Hepatitis C