Occludin Protein (OCLN) (AA 1-522) (GST tag)
(1 reference)-
- Target See all Occludin (OCLN) Proteins
- Occludin (OCLN)
- Protein Type
- Recombinant
- Protein Characteristics
- AA 1-522
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Origin
- Human
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Source
- Wheat germ
- Purification tag / Conjugate
- This Occludin protein is labelled with GST tag.
- Application
- Western Blotting (WB), ELISA, Affinity Purification (AP), Antibody Array (AA)
- Purpose
- OCLN (Human) Recombinant Protein (P01)
- Sequence
- MSSRPLESPP PYRPDEFKPN HYAPSNDIYG GEMHVRPMLS QPAYSFYPED EILHFYKWTS PPGVIRILSM LIIVMCIAIF ACVASTLAWD RGYGTSLLGG SVGYPYGGSG FGSYGSGYGY GYGYGYGYGG YTDPRAAKGF MLAMAAFCFI AALVIFVTSV IRSEMSRTRR YYLSVIIVSA ILGIMVFIAT IVYIMGVNPT AQSSGSLYGS QIYALCNQFY TPAATGLYVD QYSYHYCVVD PQEAIAIVLG FMIIVAFALI IFFAVKTRRK MDRYDKSNIL WDKEHIYDEQ PPNVEEWVKN VSAGTQDVPS PPSDYVERVD SPMAYSSNGK VNDKRFYPES SYKSTPVPEV VQELPLTSPV DDFRQPRYSS GGNFETPSKR APAKGRAGRS KRTEQDHYET DYTTGGESCD ELEEDWIREY PPITSDQQRQ LYKRNFDTGL QEYKSLQSEL DEINKELSRL DKELDDYREE SEEYMAAADE YNRLKQVKGS ADYKSKKNHC KQLKSKLSHI KKMVGDYDRQ KT
- Characteristics
- Human OCLN full-length ORF ( AAH29886, 1 a.a. - 522 a.a.) recombinant protein with GST-tag at N-terminal.
- Purification
- in vitro wheat germ expression system
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Occludin (OCLN) (AA 270-441) protein (His-IF2DI Tag)
OCLN Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Occludin (OCLN) (AA 423-522) protein (GST tag)OCLN Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Occludin (OCLN) (AA 1-522) protein (Strep Tag)Crystallography grade OCLN Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Occludin (OCLN) (AA 1-521) protein (Strep Tag)Crystallography grade OCLN Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Occludin (OCLN) protein (Myc-DYKDDDDK Tag)OCLN Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Comment
-
Preparation method: in vitro, wheat germ expression system
Product Quality tested by: 12.5% SDS-PAGE Stained with Coomassie Blue. - Restrictions
- For Research Use only
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- Buffer
- 50 mM Tris-HCI, 10 mM reduced Glutathione, pH =8.0 in the elution buffer.
- Handling Advice
- Aliquot to avoid repeated freezing and thawing.
- Storage
- -80 °C
- Storage Comment
- Best use within three months from the date of receipt of this protein.
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-
: "Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions." in: Biochimica et biophysica acta, Vol. 1860, Issue 3, pp. 516-26, (2016) (PubMed).
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: "Kiwifruit cysteine protease actinidin compromises the intestinal barrier by disrupting tight junctions." in: Biochimica et biophysica acta, Vol. 1860, Issue 3, pp. 516-26, (2016) (PubMed).
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- Target
- Occludin (OCLN)
- Alternative Name
- OCLN (OCLN Products)
- Synonyms
- AI503564 Protein, Ocl Protein, ocln Protein, oclnb Protein, tpmt Protein, OCLN Protein, wu:fd23h10 Protein, wu:fi13c01 Protein, zgc:113992 Protein, zgc:56359 Protein, BLCPMG Protein, occludin Protein, occludin S homeolog Protein, occludin a Protein, Ocln Protein, OCLN Protein, ocln.S Protein, ocln Protein, oclna Protein
- Background
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Full Gene Name: occludin
Synonyms: - Gene ID
- 4950
- Pathways
- Cell-Cell Junction Organization, Hepatitis C
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