Thrombopoietin Protein (THPO) (His tag)

Catalog No. ABIN7669820

This Recombinant Thrombopoietin protein is expressed in Escherichia coli (E. coli).

Quick Overview for Thrombopoietin Protein (THPO) (His tag) (ABIN7669820)

Target: Thrombopoietin (THPO)

Protein Type: Recombinant

Biological Activity: Inactive

Origin: Mouse

Source: Escherichia coli (E. coli)

Purity: > 95 %

Product Details

Purification tag / Conjugate: This Thrombopoietin protein is labelled with His tag.

Purpose: Recombinant Mouse TPO/Thpo protein (His tag)

Sequence: Ser 22-Phe 195

Purification: > 95 % as determined by reducing SDS-PAGE.

Sterility: 0.2 μm filtered

Endotoxin Level: < 10 EU/mg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % -8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Thrombopoietin (THPO)

Alternative Name: TPO,Thpo

Background: Thrombopoietin (TPO) is a glycoprotein hormone which belongs to the EPO/TPO family. It produced by the liver and kidney which regulates the production of platelets.Mature mouse Tpo shares 71 % and 81 % aa sequence homology with human and rat Tpo, respectively. It is an 80-85 kDa protein that consists of an N-terminal domain with homology to Erythropoietin (Epo) and a C-terminal domain that contains multiple N-linked and O-linked glycosylation sites. TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. Lineage-specific cytokine affects the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets.,C-mpl ligand,Megakaryocyte colony-stimulating factor,Megakaryocyte growth and development factor,Myeloproliferative leukemia virus oncogene ligand,THPO,Thrombopoietin

Molecular Weight:

calculated_mw: 19.0 kDa

observed_mw: 22 kDa

UniProt: P40226

Pathways: JAK-STAT Signaling, Hormone Activity