SNCA Protein (A53T, AA 1-140)

Catalog No. ABIN7827836

Recombinant SNCA protein expressed in Escherichia coli (E. coli).

Quick Overview for SNCA Protein (A53T, AA 1-140) (ABIN7827836)

Target: SNCA (Synuclein, alpha (SNCA))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by Bis-Tris PAGE,> 95 % as determined by HPLC

Product Details

Protein Characteristics: A53T, AA 1-140

Purpose: Human Alpha Synuclein (A53T) Protein

Sequence: Met1-Ala140(A53T)

Characteristics: Recombinant Human Alpha Synuclein (A53T) Protein is expressed from E.coli without tag.It contains Met1-Ala140 (A53T).

Sterility: 0.22 μm filtered

Endotoxin Level: Less than 1 EU per μg by the LAL method.

Biological Activity Comment: Human Alpha Synuclein (A53T) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.,The purity of Human Alpha Synuclein (A53T) is greater than 95% as determined by SEC-HPLC.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1 mg/mL for 500 μg or 1 mg lyophilized powder. Do not mix by vortex or vigorous shaking.

Buffer: Lyophilized from 0.22 μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.

Storage: -20 °C,-80 °C

Storage Comment: -20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3 months after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Expiry Date: 12 months

Target Details

Target: SNCA (Synuclein, alpha (SNCA))

Alternative Name: Alpha Synuclein

Background: Alpha-synuclein (α-synuclein) is a protein that is abundantly found in the brain and in a lesser amount in the heart and muscles. Its overexpression in the neurons leads to the formation of Lewy bodies that damage the dopaminergic neurons in the subtantianigra of the midbrain and leads to the progression of Parkinson's Disease (PD).

Molecular Weight: 14.49 kDa same as Bis-Tris PAGE result.

UniProt: P37840

Pathways: Synaptic Membrane, Regulation of G-Protein Coupled Receptor Protein Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Platelet-derived growth Factor Receptor Signaling, Negative Regulation of Transporter Activity, Regulation of long-term Neuronal Synaptic Plasticity